Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps 

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#Question id: 15636

#I Life Science/ Life Sciences Group – I-V

While analysing motif sequences, what is the major disadvantageous feature of PROSITE?

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#Question id: 15636

#SCPH05 I Biotechnology

While analysing motif sequences, what is the major disadvantageous feature of PROSITE?

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#Question id: 15636

#SCPH05 I Biotechnology

While analysing motif sequences, what is the major disadvantageous feature of PROSITE?

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#Question id: 15637

#SCPH01 Biochemistry

You have isolated two mutations in the Lac operon that cause constitutive expression of Lac genes. You designate these mutants Lac1– and Lac2–. Making use of an F' that carries the Lac operon with the LacY gene mutated, you construct strains that you test for both ß-galactosidase activity and Lac permease activity with results shown below.

     

Classify each mutation as dominant or recessive and as cis- or trans-acting, giving the experimental result that allows you to arrive at each conclusion. Finally, deduce what type of Lac mutation best fits the properties of Lac 1– and of Lac 2–.

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#Question id: 15637

#SCPH06 I Botany

You have isolated two mutations in the Lac operon that cause constitutive expression of Lac genes. You designate these mutants Lac1– and Lac2–. Making use of an F' that carries the Lac operon with the LacY gene mutated, you construct strains that you test for both ß-galactosidase activity and Lac permease activity with results shown below.

     

Classify each mutation as dominant or recessive and as cis- or trans-acting, giving the experimental result that allows you to arrive at each conclusion. Finally, deduce what type of Lac mutation best fits the properties of Lac 1– and of Lac 2–.

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#Question id: 15637

#SCPH28 | Zoology

You have isolated two mutations in the Lac operon that cause constitutive expression of Lac genes. You designate these mutants Lac1– and Lac2–. Making use of an F' that carries the Lac operon with the LacY gene mutated, you construct strains that you test for both ß-galactosidase activity and Lac permease activity with results shown below.

     

Classify each mutation as dominant or recessive and as cis- or trans-acting, giving the experimental result that allows you to arrive at each conclusion. Finally, deduce what type of Lac mutation best fits the properties of Lac 1– and of Lac 2–.