In frogs, maternally packaged Xwnt-11 leads to nuclear localization of _____ in the _____, which in response signals to adjacent cells to become the _____.

TLS Online TPP Program

#Question id: 9172

#SCPH06 I Botany

Two plant species live in the same biome but on different continents. Although the two species are not at all closely related, they may appear quite similar as a result of ________.

TLS Online TPP Program

#Question id: 10275

#SCPH05 I Biotechnology

In the initial phase of glycolysis, each hexose unit is phosphorylated twice and then split, producing two molecules of triose phosphate, in these reactions included irreversible reaction. How much irreversible reaction is performed in the glycolytic pathway through in this series of reactions?

TLS Online TPP Program

#Question id: 11453

#I Life Science/ Life Sciences Group – I-V

Which type of receptor would you expect to be most abundant in the antennae of a moth?

TLS Online TPP Program

#Question id: 1576

#SCPH01 Biochemistry

function of Properdin

TLS Online TPP Program

#Question id: 13088

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;