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TLS Online TPP Program
#Question id: 13065
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Agglutination of antigens by utilising specific antibodies can only occur, if
TLS Online TPP Program
#Question id: 1286
#SCPH28 | Zoology
Syndecans are cell-surface proteoglycans that:
a. bind to collagens.
b. bind to multi-adhesive matrix proteins.
c. anchor cells to the extracellular matrix.
TLS Online TPP Program
#Question id: 35
#I Life Science/ Life Sciences Group – I-V
Arrange the following molecular components of an E. coli cell in terms of their percentage of total weight cell.
TLS Online TPP Program
#Question id: 4174
#SCPH28 | Zoology
Which is not true about the genetic code?
TLS Online TPP Program
#Question id: 12911
#SCPH05 I Biotechnology
Match the following:
A. DNA polymerases i. Alkaline phosphatase a.RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase ii. T4 DNA ligase b. Thermostable DNA polymerase used in PCR
C. Ligases iii. AMV-RT c. Removes 5′-phosphates from DNA and RNA
D. Phosphatases iv. Taq DNA pol d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond