In this problem we will explore some of the many ways that mutations in two different genes can interact to produce different Mendelian ratios. Consider a hypothetical insect species that has red eyes. Imagine mutations in two different unlinked genes that can, in certain combinations, block the formation of red eye pigment yielding mutants with white eyes. In principle, there are two different possible arrangements for two biochemical steps responsible for the formation of red eye pigment. The two genes might act in series such that a mutation in either gene would block the formation of red pigment. Alternatively, the two genes could act in parallel such that mutations in both genes would be required to block the formation of red pigment.

Further complexity arises from the possibility that mutations in either gene that lead to a block in enzymatic activity could be either dominant or recessive. If the crosses is made between a wild type insect with red eyes and a true breeding white eyed strain with mutations in both genes. Such considerations yield the Pathways in series with recessive mutations in both genes, what will be the phenotype in F1 progeny?

#Question id: 14330

#SCPH05 I Biotechnology

A radioactive isotope decays at a rate proportional to the amount of isotope present. If the concentration of isotope is C (mg l^-I), its rate of decay is:

A solution of the isotope ³²p is used to radioactively label DNA for hybridisation studies. The half-life of ³²p is 14.3 days. According to institutional safety requirements, the solution cannot be discarded until the activity is 1% its present value. How long will this take?____________________

#Question id: 14331

#SCPH05 I Biotechnology

To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the Reynolds number must be at least 10⁴. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter is operated at 800 rpm. If the density of broth being stirred is close to that of water, what is the upper limit for viscosity of the suspension if adequate mixing is to be maintained?

#Question id: 14332

#SCPH05 I Biotechnology

To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the Reynolds number must be at least 10⁴. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter is operated at 800 rpm. If the density of broth being stirred is close to that of water. The mixing system is scaled up so the tank and impeller are 15 times the diameter of the laboratory equipment. The stirrer in the large vessel is operated so that the stirrer tip speed (tip speed = π N i D i) is the same as in the laboratory apparatus. How does scale-up affect the maximum viscosity. 

#Question id: 14333

#SCPH05 I Biotechnology

A cylindrical bioreactor of diameter 3 m has four baffles. A Rushton turbine mounted in the reactor has a diameter one third the tank diameter and is operated at a speed of 90 rpm. The density of the fluid is approximately I g cm^-3. The reactor is used to culture an anaerobic organism that does not require gas sparging. The broth can be assumed Newtonian. As the cells grow, the viscosity of the broth increases. when the viscosity is 1000 times greater than water, estimate the power required to achieve turbulence.

#Question id: 14337

#SCPH05 I Biotechnology

#Question id: 14337

#SCPH05 I Biotechnology