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#Question id: 447
#SCPH05 I Biotechnology
In glycolysis, fructose 1,6-bisphosphate is converted to two products with a standard free-energy change (DG'°) of 23.8 kJ/mol. Under what conditions encountered in a normal cell will the free-energy change (DG) be negative, enabling the reaction to proceed spontaneously to the right?
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#Question id: 12911
#SCPH01 Biochemistry
Match the following:
A. DNA polymerases i. Alkaline phosphatase a.RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase ii. T4 DNA ligase b. Thermostable DNA polymerase used in PCR
C. Ligases iii. AMV-RT c. Removes 5′-phosphates from DNA and RNA
D. Phosphatases iv. Taq DNA pol d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond
TLS Online TPP Program
#Question id: 16138
#SCPH01 Biochemistry
You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
Would you expect STS51 and STS52 to be present in BAC D?
TLS Online TPP Program
#Question id: 3057
#SCPH06 I Botany
Mean generation time of bacteria is 20 min. 6 hours of incubation, the bacterial population increases from 10^3 cells to
TLS Online TPP Program
#Question id: 18381
#I Life Science/ Life Sciences Group – I-V
Which bacterial strain was improved by scientists to be able to degrade 2,4-dinitrotoluene and promote plant growth