In the presence of a large excess of _______ the disulfides (cystines) are fully converted into sulfhydryls (cysteines).

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#Question id: 662

#SCPH01 Biochemistry

________ received a Nobel Prize in 1964 for determining the structure of vitamin B12. He/she also solved the structure of penicillin in 1947 and developed many techniques used in the study of large proteins.

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#Question id: 18716

#SCPH01 Biochemistry

Function of B-mercaptoethanol in SDS-PAGE is

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#Question id: 13060

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 1563

#SCPH05 I Biotechnology

A nonfunctional CD4 protein on a helper T cell would result in the helper T cell being unable to ________.

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#Question id: 562

#SCPH01 Biochemistry

Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site; this enzyme-inhibitor bond is not cleaved by the enzyme.  This is an example of what kind of inhibition?