The excision (splicing) of many group I introns requires, in addition to the primary transcript RNA:

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#Question id: 27783

#Research Methodology

An observation technique where the researcher himself actively remains associated with other members of the group and observes the behavior and activities of the group of study is known as: 

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#Question id: 16127

#SCPH01 Biochemistry

You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated  gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.

The GLN1 gene shows a rather complex regulation in response to different amino acids. When either glutamate (glu) or glutamine (gln) is added to the medium, the amount of GS expression diminished and when both glutamate and glutamine are added to the medium GS expression is shut off completely. The effects of different mutants on the response to glu and gln are shown below.

Which of the models from best fits these experimental results? Complete the model for the regulation of GLN1 that includes the effects of glu and gln.      

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#Question id: 73

#SCPH05 I Biotechnology

Match the Variation of Reaction Spontaneity (Sign of ΔG) given in Column I with the Signs of ΔH and ΔS given in column II.

COLUMN I	COLUMN II
	ΔH	ΔS	ΔG = ΔH – TΔS
1.	−	+	a. The reaction is both enthalpically and entropically opposed. It is nonspontaneous (endergonic) at all temperatures.
2.	−	−	b. The reaction is enthalpically opposed (endothermic) but entropically favored. It is spontaneous only at temperatures above T = ΔH/ΔS.
3.	+	+	c. The reaction is enthalpically favored but entropically opposed. It is spontaneous only at temperatures below T = ΔH/ΔS.
4.	+	−	d. The reaction is both enthalpically favored (exothermic) and entropically favored. It is spontaneous (exergonic) at all temperatures.

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#Question id: 633

#SCPH05 I Biotechnology

An amino acid transporter protein is responsible for the transport of a specific amino acid across a membrane. The KI values of several competitive inhibitors of the amino acid transporter are shown above. Based on these data, which of the following is most likely the amino acid transported by this protein?

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#Question id: 6994

#SCPH06 I Botany

Puberty is initiated by pulse release of _____________(hormone)