Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the operator to the far end of the operon, past the transacetylase (lacA) gene, which of the following processes would likely occur when the cell is exposed to lactose?

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#Question id: 5054

#SCPH01 Biochemistry

when one group of cells changing the behavior of an adjacent set of cells, thereby causing them to change their shape, mitotic rate, or cell fate. This kind of interaction at close range between two or more cells or tissues of different histories and properties is called as-

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#Question id: 14130

#SCPH01 Biochemistry

PAM 250 matrix is normally used for –

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#Question id: 99

#SCPH05 I Biotechnology

Which of the following are the main reasons that glycoproteins are so diverse when compared to other proteoglycans are

A) they may contain different sugars in different combinations and chain lengths.

B) β or a-glycosidic linkages may join various carbon atoms in the sugars.

C) they are found in both bacterial, plant and animal cells.

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#Question id: 3627

#SCPH01 Biochemistry

A cross is made between two plants with white flowers. All the F1 progeny had red colored flower. This is because of

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#Question id: 13088

#SCPH01 Biochemistry

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;