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TLS Online TPP Program
#Question id: 13062
#SCPH06 I Botany
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?
TLS Online TPP Program
#Question id: 4841
#SCPH12 I Genetics
The tendency of two or more than two genes to stay together during inheritance is called ___________
TLS Online TPP Program
#Question id: 942
#SCPH06 I Botany
a-ketoglutarate is a precursor for each of the following, except
TLS Online TPP Program
#Question id: 14131
#SCPH05 I Biotechnology
The suitable substitution matrix to align closely related sequences is
TLS Online TPP Program
#Question id: 2629
#SCPH28 | Zoology
Case of constitutive mutants,