The process by which developing cells achieve their functional, mature identity as liver, or muscle, or nerve is called:

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#Question id: 2990

#SCPH06 I Botany

Mitotic CDK complexes are maintained in an inactive state through inhibitory phosphorylation of the CDK subunit. Two highly conserved tyrosine and threonine residues in mammalian CDKs are subject to regulated phosphorylation.

Gene	Mutant phenotypes
A. Wee1	i. the cell does not divide but continues to grow
B. CAK kinase	ii. mitotic cyclins are inactivated
C. cdc25 phosphatase	iii. premature entry into mitosis

The following table enlists genes and phenotypes observed on mutation of these genes but not correctly matched. Which one of the following combinations is correctly matched ?

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#Question id: 2611

#I Life Science/ Life Sciences Group – I-V

Indicator of cell growth,

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#Question id: 13054

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 23248

#SCPH06 I Botany

Pyrosequencing is sequencing by synthesis whereby a PCR template is hybridised to an oligonucleotide and incubated with 

1. DNA polymerase

2.  ATP sulphurylase

3. Luciferase 

4. Apyrase

Which of the following option has correct combination?

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#Question id: 3714

#SCPH28 | Zoology

Which DNA polymerase is most responsible for chain elongation in E. coli?