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TLS Online TPP Program
#Question id: 13057
#SCPH28 | Zoology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?
TLS Online TPP Program
#Question id: 2060
#I Life Science/ Life Sciences Group – I-V
An animal cell lacking carbohydrates on the external surface of its plasma membrane would likely be impaired in which function?
TLS Online TPP Program
#Question id: 2625
#SCPH28 | Zoology
positive regulation is applied when
TLS Online TPP Program
#Question id: 84
#I Life Science/ Life Sciences Group – I-V
Below is the Fischer projection of D-galactose. Which is the proper Haworth projection of β-D-galactopyranose?
TLS Online TPP Program
#Question id: 5558
#I Life Science/ Life Sciences Group – I-V
Fertilization of an egg without activation is most like ________.