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TLS Online TPP Program
#Question id: 13057
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?
TLS Online TPP Program
#Question id: 12912
#I Life Science/ Life Sciences Group – I-V
Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program
#Question id: 1654
#SCPH05 I Biotechnology
The low affinity FcγRII IgG receptor:
TLS Online TPP Program
#Question id: 11756
#SCPH28 | Zoology
A 65-year-old man has a heart attack and experiences
cardiopulmonary arrest while being transported to the emergency room. The
following laboratory values are obtained from arterial blood:
plasma pH = 7.12,
plasma PCO2 = 60mm Hg, and
plasma HCO3− concentration = 19 mEq/L.
Which of the following best describes his acid-base disorder?
TLS Online TPP Program
#Question id: 3011
#I Life Science/ Life Sciences Group – I-V
Bacteria that may form endospores include