How the GA3 is differ from GA1______

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#Question id: 7702

#SCPH06 I Botany

If organisms A, B, and C belong to the same class but to different orders and if organisms C, D, and E belong to the same order but to different families, which of the following pairs of organisms would be expected to show the greatest degree of structural homology?

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#Question id: 43

#SCPH28 | Zoology

If 10 g of ice at 0°C is converted to water at the same temperature, the change in entropy will be- (latent heat 80 cal/g

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#Question id: 11082

#SCPH28 | Zoology

A 67-year-old man has a solid tumor that pushes against an airway partially obstructing air flow to the distal alveoli. Which point on the ventilation-perfusion line of the O2-CO2 diagram corresponds to the alveolar gas of these distal alveoli?

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#Question id: 13093

#SCPH06 I Botany

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.

 

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#Question id: 18621

#SCPH01 Biochemistry

Variants of PCR has been devised to enhance efficiency are

a) qPCR with SYBR, TaqMan and MB

b) nested PCR

c) Touchdown start PCR

d) hot start PCR