Why is it preferable to use large computers and databases in searching for individual genes, rather than testing each sequence for possible function?

TLS Online TPP Program

#Question id: 12920

#SCPH01 Biochemistry

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.

TLS Online TPP Program

#Question id: 15620

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition Lac– strain, called lac3–, is linked to the Tn5 insertion. From a strain carrying the Tn5 insertion and lac3– mutation you isolate an F’ that caries a region of the chromosome that includes both Tn5 and the linked Lac region. Introduce this F’ into an F– strain carrying lac1– by selecting for Kanr. These merodiploids express ß-galactosidase normally. What does this result tell you about the relationship between the lac3– and lac1- mutations?  

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#Question id: 3514

#SCPH06 I Botany

Which of the following are not conclude of that monohybrid cross?

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#Question id: 431

#SCPH06 I Botany

Which is not generally a molecule used to conserve energy?

TLS Online TPP Program

#Question id: 15152

#SCPH01 Biochemistry

In this problem we will explore some of the many ways that mutations in two different genes can interact to produce different Mendelian ratios. Consider a hypothetical insect species that has red eyes. Imagine mutations in two different unlinked genes that can, in certain combinations, block the formation of red eye pigment yielding mutants with white eyes. In principle, there are two different possible arrangements for two biochemical steps responsible for the formation of red eye pigment. The two genes might act in series such that a mutation in either gene would block the formation of red pigment. Alternatively, the two genes could act in parallel such that mutations in both genes would be required to block the formation of red pigment.

Further complexity arises from the possibility that mutations in either gene that lead to a block in enzymatic activity could be either dominant or recessive. If the crosses is made between a wild type insect with red eyes and a true breeding white eyed strain with mutations in both genes. Such considerations yield the Pathways in series with dominant mutations in both genes, determine the phenotype of the F1 progeny and the expected phenotypic ratio of red to white eyed insects in the F2.