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TLS Online TPP Program
#Question id: 17073
#SCPH01 Biochemistry
Hypoxanthine is converted into guanine by the enzyme HGPRT an enzymes of the salvage pathway. What does HGPRT stands for?
TLS Online TPP Program
#Question id: 13054
#SCPH06 I Botany
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program
#Question id: 18216
#SCPH05 I Biotechnology
Which of the following is not correct about FASTA?
TLS Online TPP Program
#Question id: 971
#SCPH28 | Zoology
What is the source of the nitrogen atoms 3 and 9 during the de novo biosynthesis of purines?
TLS Online TPP Program
#Question id: 561
#I Life Science/ Life Sciences Group – I-V
Enzyme X exhibits maximum activity at pH = 6.9. X shows a fairly sharp decrease in its activity when the pH goes much lower than 6.4. One likely interpretation of this pH activity is that: