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TLS Online TPP Program
#Question id: 13057
#SCPH28 | Zoology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?
TLS Online TPP Program
#Question id: 10567
#SCPH06 I Botany
Cell A is having solute potential of -14 and Pressure potential of 3. Cell B is having Solute potential of -16 and Pressure potential of 4. The water will travel from
TLS Online TPP Program
#Question id: 15182
#SCPH01 Biochemistry
Which of the following types of effect are responsible for lowering the efficiency of electrophoresis?
TLS Online TPP Program
#Question id: 14746
#SCPH05 I Biotechnology
During cultivation of microorganism in a fermenter, various parameters are controlled by appropriate mechanism, match the following
TLS Online TPP Program
#Question id: 3004
#SCPH28 | Zoology
Bacterium was inoculated into a rich medium. If at the end of 5 hours the total number of cells is 1024, then the number of elapsed generations and the generation time respectively is