Ecosystem services include processes that increase the quality of the abiotic environment. Which of the following processes would fall under this category?

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#Question id: 11136

#I Life Science/ Life Sciences Group – I-V

Most of the neurons in the human central nervous system are ________.

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#Question id: 6948

#SCPH01 Biochemistry

Which of the following genes is expressed at the posterior margin of each parasegment

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#Question id: 10892

#SCPH06 I Botany

Considering environment and ecosystem, identify the INCORRECT statement

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#Question id: 7759

#SCPH01 Biochemistry

WRKY plant-specific group of transcription factors that play important role in many plant metabolic and developmental processes such as;

a) It contain a 30 amino acid region named for the conserved amino acid sequence WRKYGQK in its N-terminal domain

b) It is an important regulators of plant–pathogen interactions as well as senescence

c) WRKY53 is involved in the regulation of dark-induced leaf senescence

d) In Arabidopsis, leaf senescence is delayed in knockout mutants of the WRKY53 gene

e) WRKY53 also binds to the promoter of the WRKY53 gene, inhibiting its own expression in a negative feedback loop

Which of the following given prediction about WRKY transcription factor is correct?

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#Question id: 13094

#SCPH28 | Zoology

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                     

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.

 

a) Pattern-1, DV only + Ligase→No colonies b/c you have digested with 2 different restriction enzymes that can’t ligate together 

b) Pattern-2, DYG only + Ligase→ No colonies because all you transformed is the digested, linear yeast DNA.

c) Pattern-3, Water + Ligase→ No plasmid with the ampicillin resistance gene (or any DNA) was transformed into the bacteria and so it won’t grow in the presence of ampicillin.

d)Pattern-3, DV + DYG + Ligase→Colonies. The plasmid and yeast gene can ligate together to form a functional plasmid that will express the ampicillin resistance gene.

e) Pattern-1 and 2 only, DV + DYG (No Ligase) →No colonies because, although you have both digested plasmid and a digested yeast gene with complementary sticky ends

Which of the following statements about these ligations and their pattern is correct?