What strategy was used to rescue Illinois prairie chickens from a recent extinction vortex?

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#Question id: 3628

#SCPH28 | Zoology

Two mutant plants, both bearing white flowers, were crossed. All F1 plants had red colored flowers. Which one of the following conclusions is correct?

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#Question id: 13038

#SCPH28 | Zoology

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

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#Question id: 18889

#SCPH01 Biochemistry

In light microscopy, which of the following is used as fixatives prior to staining technique?

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#Question id: 13089

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

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#Question id: 8932

#SCPH28 | Zoology

What is the correct sequence of the following four events during an animalʹs development?

1. gastrulation

2. metamorphosis

3. fertilization

4. cleavage