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TLS Online TPP Program
#Question id: 13055
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?
TLS Online TPP Program
#Question id: 12935
#SCPH28 | Zoology
The integrity of the RNA should always be checked by _____
TLS Online TPP Program
#Question id: 4124
#SCPH01 Biochemistry
Assuming that the average amino acid residue contributes 110 to the peptide molecular weight, what will be the minimum length of the mRNA encoding a protein of molecular weight 50,000?
TLS Online TPP Program
#Question id: 12683
#SCPH05 I Biotechnology
Which is the full complement of DNA from an Organism, carrying all the information needed to specify the structure of every protein?
TLS Online TPP Program
#Question id: 10246
#SCPH28 | Zoology
Under an ideal free distribution: