According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 17076

#SCPH28 | Zoology

The various advantages of serum-free media could be

1. Improved reproducibility of results from different laboratories

2. Easier downstream processing of products from cultured cells

3. Biassays are free from interference due to serum proteins

4. They permit selective culture of diffrentiated cell types from the heterogeneous cultures

Which of the statements are true?

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#Question id: 19514

#SCPH05 I Biotechnology

A cathode and an anode are the most common components of an electrochemical cell. Which of the following claims about the cathode is correct?

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#Question id: 10809

#SCPH05 I Biotechnology

Sieve element sap is rich in sugars and other organic molecules, These molecules represent an energy investment for the plant. How their loss be prevented when sieve elements are damaged?

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#Question id: 5125

#SCPH01 Biochemistry

In above experiment in figure.A two differently positioned blastula cells are specified to become distinct muscle and neuronal cells.In figure.B when These two different blastula cells are placed together in culture. Following results are obtained according to step(1) and step(2). Which statement is correct about muscle cell commitment?

 

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#Question id: 1552

#SCPH01 Biochemistry

A common clinical pathological finding during a viral infection is