According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 14213

#SCPH05 I Biotechnology

Which phase has the condition of specific growth rate “μ = 0”?

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#Question id: 14213

#SCPH05 I Biotechnology

Which phase has the condition of specific growth rate “μ = 0”?

TLS Online TPP Program

#Question id: 14214

#SCPH05 I Biotechnology

Viscosity ranges for different impeller, find correct match?

      Types                                                                         Maximum viscosity(cp)

      P) Helical ribbon                                (I) 105

      Q) Propellers                                                                    (II)  106

      R) Helical screws                                                             (III) 104

TLS Online TPP Program

#Question id: 14214

#SCPH05 I Biotechnology

Viscosity ranges for different impeller, find correct match?

      Types                                                                         Maximum viscosity(cp)

      P) Helical ribbon                                (I) 105

      Q) Propellers                                                                    (II)  106

      R) Helical screws                                                             (III) 104

TLS Online TPP Program

#Question id: 14215

#SCPH05 I Biotechnology

kLa is measured in dynamic method of oxygen transfer by 

TLS Online TPP Program

#Question id: 14215

#SCPH05 I Biotechnology

kLa is measured in dynamic method of oxygen transfer by