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#Question id: 9570
#SCPH06 I Botany
Which of the following would not be a significant component of the energy budget of a person sitting on a park bench on a warm and windy night,
exposed to the clear night sky?
TLS Online TPP Program
#Question id: 15495
#SCPH05 I Biotechnology
Calculate the degree of reduction for following substrates:
A) Methane
B) Glucose
C) Ethanol
TLS Online TPP Program
#Question id: 1568
#I Life Science/ Life Sciences Group – I-V
which one of these is not a characteristics of B-cell?
TLS Online TPP Program
#Question id: 13089
#SCPH28 | Zoology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 2’s plasmid is;
TLS Online TPP Program
#Question id: 9192
#SCPH06 I Botany
The oak tree pathogen Phytophthora ramorum has migrated 650 km in ten years. West Nile virus spread from New York State to 46 others states in five years. The difference in the rate of spread is probably related to