Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

#Question id: 11025

#I Life Science/ Life Sciences Group – I-V

Listed below are the hydrostatic and oncotic pressures within a microcirculatory bed:

Plasma colloid osmotic pressure = 25 mm Hg

Capillary hydrostatic pressure = 25 mm Hg

Venous hydrostatic pressure = 5 mm Hg

Arterial pressure = 80 mm Hg

Interstitial fluid hydrostatic pressure = −5 mm Hg

Interstitial colloid osmotic pressure = 10 mm Hg

Capillary filtration coefficient = 10 ml/min/mm Hg

What is the rate of net fluid movement across the capillary wall?

#Question id: 11026

#I Life Science/ Life Sciences Group – I-V

Listed below are the hydrostatic and oncotic pressures and filtration rate across a muscle capillary wall:

Capillary hydrostatic pressure (Pc) = 25 mm Hg

Plasma colloid osmotic pressure (Πp) = 25 mm Hg

Interstitial colloid osmotic pressure (ΠI) = 10 mm Hg

Interstitial hydrostatic pressure (PI) = −5 mm Hg

Capillary filtration rate = 150 ml/min

What is the capillary filtration coefficient?

#Question id: 11027

#I Life Science/ Life Sciences Group – I-V

Which of the following is the correct sequence of blood flow in birds and mammals?

#Question id: 11059

#I Life Science/ Life Sciences Group – I-V

A healthy, 45-year-old man is reading the newspaper. Which of the following muscles are used for quiet breathing?

#Question id: 11059

#SCPH28 | Zoology

A healthy, 45-year-old man is reading the newspaper. Which of the following muscles are used for quiet breathing?

#Question id: 11060

#I Life Science/ Life Sciences Group – I-V

A healthy, 25-year-old medical student participates in a 10-km charity run for the American Heart Association. Which of the following muscles does the student use (contract) during expiration?