Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 1391

#SCPH05 I Biotechnology

If an adult person has a faulty version of the human analog to ced-4 of the nematode, which of the following is most likely to result?

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#Question id: 4327

#SCPH28 | Zoology

What would occur if the repressor of an inducible operon were mutated so it could not bind the operator?

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#Question id: 3530

#SCPH01 Biochemistry

Mendel’s law of segregation, as applied to the behavior of chromosomes in meiosis, means that:

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#Question id: 1543

#I Life Science/ Life Sciences Group – I-V

Effector  cytokines of TH 1 cells?

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#Question id: 18007

#SCPH28 | Zoology

The following geological eras mark the advent of important events in the history of earth- origin of first vertebrates armored fishes, origin of First reptiles, origin of first dinosaurs;

a) Triassic b) Pennsylvanian

c) Ordovician d) Cambrian

Identify the correct match of the events with the geological era;