TLS Online TPP Program

#Question id: 13056


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
 Which of the following is true for traditional and real time PCR?

#SCPH06 I Botany
  1. Both the techniques allow detection of the product in early stages
  2. Real time PCR can detect the product in early stages as compared to traditional PCR
  3. Both systems require different primers
  4. Both techniques are end point product determination methods

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TLS Online TPP Program

#Question id: 5701

#SCPH06 I Botany

Cross-over suppressors are production of gametes having cross over products is lethal. Which of the following statement is incorrect?

TLS Online TPP Program

#Question id: 5701

#SCPH28 | Zoology

Cross-over suppressors are production of gametes having cross over products is lethal. Which of the following statement is incorrect?

TLS Online TPP Program

#Question id: 5702

#SCPH06 I Botany

What is probability of couple where male is karyotypically normal and the female in pericentric inversion in heterozygous condition produced child with disability if no crossing over takes placed within pericentric inversion. (Note: Duplication and deletion in gamete will results  in disability)

TLS Online TPP Program

#Question id: 5702

#SCPH28 | Zoology

What is probability of couple where male is karyotypically normal and the female in pericentric inversion in heterozygous condition produced child with disability if no crossing over takes placed within pericentric inversion. (Note: Duplication and deletion in gamete will results  in disability)

TLS Online TPP Program

#Question id: 5703

#SCPH06 I Botany

Robertsonian translocation is reciprocal exchange of genetic segment between two acrocentric chromosomes. Which of the following is incorrect statement?

TLS Online TPP Program

#Question id: 5703

#SCPH28 | Zoology

Robertsonian translocation is reciprocal exchange of genetic segment between two acrocentric chromosomes. Which of the following is incorrect statement?