Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 19208

#SCPH28 | Zoology

Nicotinic acid detection in whole cell biosensor can be done using 

TLS Online TPP Program

#Question id: 15534

#SCPH05 I Biotechnology

In FASTA, For a Z-score > 15, the match can be considered extremely ______ with _____ of a homologous relationship.

TLS Online TPP Program

#Question id: 6969

#SCPH28 | Zoology

In Xenopus, the elongation of the mesoderm toward the anterior results from the intercalation of cells during a process called:

TLS Online TPP Program

#Question id: 10521

#SCPH05 I Biotechnology

Which is an example of a plant-derived DAMP found in tomato that is produced in response to wounding associated with herbivore activity?

TLS Online TPP Program

#Question id: 671

#SCPH05 I Biotechnology

Which was a major scientific accomplishment by Linus Pauling?