Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 5742

#SCPH06 I Botany

When an allotetraploid (AABB) is backcrossed to one of its progenitor species (AA), a sterile progeny is produced.  The genomic composition of this sterile individual can be best represented by

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#Question id: 4341

#SCPH28 | Zoology

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would be most likely?

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#Question id: 4304

#SCPH01 Biochemistry

Using codon table, identify a 5′ → 3′ sequence of nucleotides in the DNA template strand for an mRNA coding for the polypeptide sequence Phe-Pro-Lys.

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#Question id: 11135

#SCPH28 | Zoology

The three stages of information processing in animals include ________.

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#Question id: 9259

#SCPH28 | Zoology

The oceans affect the biosphere in all of the following ways except