Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

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#Question id: 13042

#SCPH01 Biochemistry

For analyzing DNA sequences, two techniques have been developed, one is _________ method frequently termed_________, involving the enzymatic synthesis of DNA and stopping the synthesis at specific nucleotides. While another technique, the __________ method of DNA sequencing developed by ___________ is often used for sequencing small fragments of DNA.

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#Question id: 9218

#SCPH06 I Botany

What strategy was used to rescue Illinois prairie chickens from a recent extinction vortex?

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#Question id: 6957

#SCPH06 I Botany

The effect of grafting an extra piece of notochord into a dorsal position adjacent to pre-somitic mesoderm is

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#Question id: 10592

#SCPH28 | Zoology

Given below are the steps to assess the population size of grasshoppers in a given area, the size of the population is estimated as follows

Which of the following correct combination of parameter?

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#Question id: 23171

#SCPH06 I Botany

Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize