Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.

Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.

II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 5059

#SCPH28 | Zoology

The responding tissue has already been specified and needs only an environment that allows the expression of these traits, process called as

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#Question id: 2130

#SCPH06 I Botany

Peripheral membrane proteins:

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#Question id: 1631

#I Life Science/ Life Sciences Group – I-V

Specific antibodies are readily detectable in serum following primary contact with antigen after:

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#Question id: 16126

#SCPH06 I Botany

2 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS, that could explain the effect of the gln2– and gln3– mutations on the regulation of GLN1.

The GLN1 gene shows a rather complex regulation in response to different amino acids. When either glutamate (glu) or glutamine (gln) is added to the medium, the amount of GS expression diminished and when both glutamate and glutamine are added to the medium GS expression is shut off completely. The effects of different mutants on the response to glu and gln are shown below.

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#Question id: 10537

#I Life Science/ Life Sciences Group – I-V

Given below some functions of the glutamate receptor-like (GLR) genes. Which one is incorrect?