To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

#Question id: 7303

#SCPH01 Biochemistry

The ‘quartet model’ explains how the protein products of the ABCE-function genes might interact to control floral organ identity. Following statements are based on this model. Which one of them is incorrect?

#Question id: 7303

#I Life Science/ Life Sciences Group – I-V

The ‘quartet model’ explains how the protein products of the ABCE-function genes might interact to control floral organ identity. Following statements are based on this model. Which one of them is incorrect?

#Question id: 7303

#SCPH06 I Botany

The ‘quartet model’ explains how the protein products of the ABCE-function genes might interact to control floral organ identity. Following statements are based on this model. Which one of them is incorrect?

#Question id: 7303

#SCPH28 | Zoology

The ‘quartet model’ explains how the protein products of the ABCE-function genes might interact to control floral organ identity. Following statements are based on this model. Which one of them is incorrect?

#Question id: 7304

#SCPH01 Biochemistry

Which one of the following will be observed when auxin to cytokinin ratio is increased in the culture medium during organogenesis from tobacco pith callus?

#Question id: 7304

#I Life Science/ Life Sciences Group – I-V

Which one of the following will be observed when auxin to cytokinin ratio is increased in the culture medium during organogenesis from tobacco pith callus?