To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 23254

#SCPH01 Biochemistry

FISH or GISH is used to _____ and ______ the presence or absence of specific DNA sequences on chromosomes.

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#Question id: 10949

#I Life Science/ Life Sciences Group – I-V

Which of the following applies to AIDS patients?

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#Question id: 3525

#SCPH01 Biochemistry

In a plant, red fruit (R) is dominant over yellow fruit (r) and tallness (T) is dominant over shortness (t). If a plant with RRTt genotype was crossed with rrtt plant results progeny, are

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#Question id: 8704

#SCPH28 | Zoology

To apply the principle of maximum parsimony to construction of a phylogenetic tree, ________.

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#Question id: 267

#SCPH12 I Genetics

Which of the following is mismatched?