To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

#Question id: 2634

#SCPH28 | Zoology

Tryptophan, the end-product of the enzymes encoded by the trp operon, therefore acts as a co-repressor and inhibits its own synthesis through:

#Question id: 7309

#SCPH01 Biochemistry

Following statements are regarding to the molecular basis of the coincidence model in Arabidopsis.

A. In Arabidopsis under short days, there is little overlap between CO mRNA expression and daylight.

B. CO protein does not accumulate to sufficient levels in the phloem to promote the expression of the transmissible floral stimulus and the plant remains vegetative.

C. Under long days, the peak of CO mRNA abundance (at hours 12 through 16) overlaps with daylight (sensed by phyA and cryptochrome [cry]), allowing CO protein to accumulate.

D. CO activates FLC mRNA expression in the phloem, which causes flowering when the FT protein is translocated to the apical meristem.

Which of the following is incorrect?

#Question id: 12930

#SCPH01 Biochemistry

#Question id: 11026

#I Life Science/ Life Sciences Group – I-V

Listed below are the hydrostatic and oncotic pressures and filtration rate across a muscle capillary wall:

Capillary hydrostatic pressure (Pc) = 25 mm Hg

Plasma colloid osmotic pressure (Πp) = 25 mm Hg

Interstitial colloid osmotic pressure (ΠI) = 10 mm Hg

Interstitial hydrostatic pressure (PI) = −5 mm Hg

Capillary filtration rate = 150 ml/min

What is the capillary filtration coefficient?

#Question id: 20982

#SCPH28 | Zoology