To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

#Question id: 5536

#SCPH28 | Zoology

Which of the following is not related with the spot of invagination during gastrulation?

#Question id: 10689

#SCPH06 I Botany

Which of the following is correct statement?

A) Body size of parasitoid is always greater than host but parasite usually smaller than host

B) Predator immediately kill their prey however parasitoid never kill their host

C) Intrinsic rate of parasite is faster than host but intrinsic rate of predator is slower than prey

D) Body association time between parasite and host is more than time association between predator and prey

#Question id: 15141

#SCPH05 I Biotechnology

#Question id: 5041

#SCPH01 Biochemistry

Which one of the following developmental processes in animals is not dependent on cellular movements?

#Question id: 8701

#SCPH28 | Zoology

The question refers to the following table, which compares the percent sequence homology of four different parts (two introns and two exons) of a gene that is found in five different eukaryotic species. Each part is numbered to indicate its distance from the promoter (for example, Intron I is the one closest to the promoter). The data reported for species A were obtained by comparing DNA from one member of species A to another member of species A.

Based on the tabular data, and assuming that time advances vertically, which phylogenetic tree is the most likely depiction of the evolutionary relationships among these five species?