To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

#Question id: 11078

#SCPH28 | Zoology

A child has been eating round candies approximately 1 and 1.5 cm in diameter and inhaled one down his airway blocking his left bronchiole. Which of the following will describe the changes that occur?

#Question id: 11079

#I Life Science/ Life Sciences Group – I-V

The diffusing capacity of a gas is the volume of gas that will diffuse through a membrane each minute for a pressure difference of 1 mm Hg. Which of the following gases is often used to estimate the oxygen diffusing capacity of the lungs?

#Question id: 11079

#SCPH28 | Zoology

The diffusing capacity of a gas is the volume of gas that will diffuse through a membrane each minute for a pressure difference of 1 mm Hg. Which of the following gases is often used to estimate the oxygen diffusing capacity of the lungs?

#Question id: 11080

#I Life Science/ Life Sciences Group – I-V

The O2-CO2 diagram here shows a ventilation-perfusion ratio line for the normal lung. Which of the following best describes the effect of decreasing ventilation-perfusion ratio on the alveolar Po2 and Pco2?

#Question id: 11080

#SCPH28 | Zoology

The O2-CO2 diagram here shows a ventilation-perfusion ratio line for the normal lung. Which of the following best describes the effect of decreasing ventilation-perfusion ratio on the alveolar Po2 and Pco2?

#Question id: 11081

#I Life Science/ Life Sciences Group – I-V

In which of the following conditions is alveolar Po2 increased and alveolar Pco2 decreased?