To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

#Question id: 10506

#SCPH05 I Biotechnology

Plant growth promoting rhizobacteria (PGPRs) such as Pseudomonas aeruginosa, that alleviate plant abiotic or biotic stress in soil by releasing of

#Question id: 10506

#SCPH06 I Botany

Plant growth promoting rhizobacteria (PGPRs) such as Pseudomonas aeruginosa, that alleviate plant abiotic or biotic stress in soil by releasing of

#Question id: 10507

#I Life Science/ Life Sciences Group – I-V

Rhizobacteria function as

 a) Nitrogen fixation

 b) depositing pathogens directly into the vascular system,

 c) producing antibiotics and antifungal agents for inhibiting soil pathogens

 d) herbivore sensors by sending electrical or chemical signals to surrounding cells

 Which one of the following combination is correct?

#Question id: 10507

#I Life Science/ Life Sciences Group – I-V

Rhizobacteria function as

 a) Nitrogen fixation

 b) depositing pathogens directly into the vascular system,

 c) producing antibiotics and antifungal agents for inhibiting soil pathogens

 d) herbivore sensors by sending electrical or chemical signals to surrounding cells

 Which one of the following combination is correct?

#Question id: 10507

#SCPH05 I Biotechnology

Rhizobacteria function as

 a) Nitrogen fixation

 b) depositing pathogens directly into the vascular system,

 c) producing antibiotics and antifungal agents for inhibiting soil pathogens

 d) herbivore sensors by sending electrical or chemical signals to surrounding cells

 Which one of the following combination is correct?

#Question id: 10507

#SCPH05 I Biotechnology

Rhizobacteria function as

 a) Nitrogen fixation

 b) depositing pathogens directly into the vascular system,

 c) producing antibiotics and antifungal agents for inhibiting soil pathogens

 d) herbivore sensors by sending electrical or chemical signals to surrounding cells

 Which one of the following combination is correct?