To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 20000

#SCPH28 | Zoology

Example of Cross-reactivity

a) Dominant and recessive gene

b) Autoimmune disease

c) Blood group mismatch

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#Question id: 2925

#SCPH05 I Biotechnology

Mitotic cyclin levels in early Xenopus embryos are regulated by:

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#Question id: 937

#I Life Science/ Life Sciences Group – I-V

Glutamate dehydrogenase has different roles in different organisms. Which of these listed is mismatched?

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#Question id: 1541

#SCPH01 Biochemistry

Nonphagocytic cell of immune system,

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#Question id: 7311

#SCPH06 I Botany

According to the ABC model of flower development in Arabidopsis, three classes of organ identity genes— designated A, B, and C— are required to specify the identity of floral organs in each whorl of a flower. If wild type Arabidopsis were transformed with a chimeric gene composed of a C class promoter fused to a B class coding sequence, which of the following arrangements   (outer to inner) would be predicted?