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TLS Online TPP Program

#Question id: 13097

You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.
 
If you take any bacterial gene sequence, before you begin doing any sequence analysis on it, there are six potential open reading frames. Why are there six?

#SCPH06 I Botany
  1. There are 3 reading frames on each of two strands, because the top strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT and If the bottom strand looked like the mRNA, then the reading frame could start with a TGT, a GTT, or a TTC.
  2. There are 6 reading frames on each of two strands because the strand look like the mRNA, then the reading frame could start with a CCC, a CCG, a CGT, a GTA, TAC or a ACG.
  3. There are 3 reading frame only on the top of the strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT.
  4. There are 3 reading frames on each of two strands, because the top strand looked like the mRNA, then the reading frame could start with a CCC, a CCG, or a CGT and If the bottom strand looked like the mRNA, then the reading frame could start with a GGG, a GGC, or a GCA.
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TLS Online TPP Program

#Question id: 5544

#SCPH01 Biochemistry

Which embryonic membrane forms the commonly of the placenta?
TLS Online TPP Program

#Question id: 13055

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?
TLS Online TPP Program

#Question id: 4596

#SCPH06 I Botany

In a hypothetical environment, fishes called pike-cichlids are visual predators of large, adult algae-eating fish (in other words, they locate their prey by sight). The population of algae-eaters experiences predatory pressure from pike-cichlids. Which of the following is least likely to result in the algae-eater population in future generations?
TLS Online TPP Program

#Question id: 12748

#SCPH01 Biochemistry

Name the technique which uses an enzyme that produces a colorimetric or luminescence product.
TLS Online TPP Program

#Question id: 19682

#SCPH05 I Biotechnology

Which of the following statements concerning methylamine is correct?