TLS Online TPP Program

#Question id: 15223


Which of the following method is particularly useful for separating isoenzymes 

#SCPH06 I Botany
  1. SDS-PAGE
  2. Agarose gel
  3. IEF
  4. Gradient gel
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TLS Online TPP Program

#Question id: 10284

#I Life Science/ Life Sciences Group – I-V

Allosteric regulation of enzyme multisubunit or multiactive site shows cooperativity with the sigmoidal graph between substerate conc. v/s Activity,


a) Cell contain NADH, FADH2 and ATP, that inhibits the allosteric enzyme activity by lowering the affinity with its substate, graph shifts towards B from control

 b) A- shows low ATP, the Km for fructose 6-phosphate is relatively low, the affinity of the enzyme with its substate is relatively high

 c) Haemoglobin, Phosphofructokinase, Phosphate dehydrogenase complex, α-ketoglutarate dehydrogenase complex, these all enzymes shows characteristics of sigmoidal graph

 d) When ATP is high, Km for fructose 6-phosphate is greatly increased- this activity shown by B in the graph

 e) fructose 2,6-bisphosphate binds to its allosteric site on PFK-1, it increases the enzyme’s affinity for its substrate fructose 6-phosphate and reduces its affinity for the allosteric inhibitors ATP and citrate- shown in graph by A

 Which of the following  combination of the enzyme activity with its substate correctly shown;

TLS Online TPP Program

#Question id: 16135

#SCPH06 I Botany

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
Would you expect the DNA sequence of PCR product obtained at STS5 using mouse genomic DNA as template to more closely resemble that obtained using BAC B, BAC D, or BAC F? Briefly explain your answer.

TLS Online TPP Program

#Question id: 7767

#SCPH28 | Zoology

How can evolution select for phenotypes that postpone reproduction or sexual maturity?” There is often a trade-off between reproduction and maintenance, and in many species reproduction and senescence are closely linked. Recent studies of mice, Caenorhabditis elegans, and Drosophila suggest that there is a conserved genetic pathway that regulates aging and that it can indeed be selected for. This pathway involves the response to insulin and insulin-like growth factors, Name of these transcription factors are

TLS Online TPP Program

#Question id: 3052

#SCPH05 I Biotechnology

How many average CFU will form on petriplate if 0.1ml sample plating from 104 diluted sample (original sample contain 3x10^7 bacteria per ml)

TLS Online TPP Program

#Question id: 12725

#SCPH05 I Biotechnology

Match the following

DNA form

Helix Direction

Number of bases per helix turn

1. B

a. Left Handed

i. 10

2.  A

b. Right Handed

ii. 12

3.  C

c. Left Handed

iii. 14

4. Z

d. Right Handed

iv. 9