Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition  isolate  a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for   Kanr   transductants. In this case, all 100   Kanr   transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?

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#Question id: 18661

#SCPH01 Biochemistry

Which of the following highly sensitive analytical technique particularly useful for studying microheterogeneity in a protein

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#Question id: 18661

#SCPH06 I Botany

Which of the following highly sensitive analytical technique particularly useful for studying microheterogeneity in a protein

TLS Online TPP Program

#Question id: 18662

#SCPH01 Biochemistry

Which of the following method is particularly useful for separating isoenzymes, which are different forms of the same enzyme, often differing by only one or two amino-acid residues.

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#Question id: 18662

#SCPH06 I Botany

Which of the following method is particularly useful for separating isoenzymes, which are different forms of the same enzyme, often differing by only one or two amino-acid residues.

TLS Online TPP Program

#Question id: 18663

#SCPH01 Biochemistry

When a protein exists in mono-, di- and tri-phosphorylated forms. The difference of a couple of phosphate groups has no significant effect on the overall relative molecular mass of the protein, hence a single band on SDS gels, but the small charge difference introduced on each molecule can be detected by

TLS Online TPP Program

#Question id: 18663

#SCPH06 I Botany

When a protein exists in mono-, di- and tri-phosphorylated forms. The difference of a couple of phosphate groups has no significant effect on the overall relative molecular mass of the protein, hence a single band on SDS gels, but the small charge difference introduced on each molecule can be detected by