You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. Next you isolate a mutation, designated MelB–, which gives uninducible melibiase activity. Mapping experiments show that MelB– is linked to Mel1. Using an F' factor that carries the chromosomal region surrounding Mel1, you perform the following genetic tests:

                      

Describe the basic genetic properties of the MelB– mutation?

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#Question id: 15225

#SCPH28 | Zoology

Which electrophoresis used to separate complete chromosome

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#Question id: 15226

#SCPH01 Biochemistry

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

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#Question id: 15226

#SCPH05 I Biotechnology

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

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#Question id: 15226

#SCPH06 I Botany

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

TLS Online TPP Program

#Question id: 15226

#SCPH28 | Zoology

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

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#Question id: 15227

#SCPH01 Biochemistry

Electrophoresis of RNA is usually carried out in