2 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS, that could explain the effect of the gln2– and gln3– mutations on the regulation of GLN1.

The GLN1 gene shows a rather complex regulation in response to different amino acids. When either glutamate (glu) or glutamine (gln) is added to the medium, the amount of GS expression diminished and when both glutamate and glutamine are added to the medium GS expression is shut off completely. The effects of different mutants on the response to glu and gln are shown below.

TLS Online TPP Program

#Question id: 15483

#SCPH06 I Botany

A novel technique for strain improvement combining the advantage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes.

TLS Online TPP Program

#Question id: 15483

#SCPH28 | Zoology

A novel technique for strain improvement combining the advantage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes.

TLS Online TPP Program

#Question id: 15484

#SCPH01 Biochemistry

Microbial process produces l-form of amino acids which makes the process advantageous as compared to synthetic processes. Strains employed for amino acid 1. 1. 1. production are 

1. wild-type strains 

2. auxotrophic mutants

3. regulatory mutants

4. auxotrophic regulatory mutants

Which of the following combination has correct options

TLS Online TPP Program

#Question id: 15484

#I Life Science/ Life Sciences Group – I-V

Microbial process produces l-form of amino acids which makes the process advantageous as compared to synthetic processes. Strains employed for amino acid 1. 1. 1. production are 

1. wild-type strains 

2. auxotrophic mutants

3. regulatory mutants

4. auxotrophic regulatory mutants

Which of the following combination has correct options

TLS Online TPP Program

#Question id: 15484

#SCPH05 I Biotechnology

Microbial process produces l-form of amino acids which makes the process advantageous as compared to synthetic processes. Strains employed for amino acid 1. 1. 1. production are 

1. wild-type strains 

2. auxotrophic mutants

3. regulatory mutants

4. auxotrophic regulatory mutants

Which of the following combination has correct options

TLS Online TPP Program

#Question id: 15484

#SCPH06 I Botany

Microbial process produces l-form of amino acids which makes the process advantageous as compared to synthetic processes. Strains employed for amino acid 1. 1. 1. production are 

1. wild-type strains 

2. auxotrophic mutants

3. regulatory mutants

4. auxotrophic regulatory mutants

Which of the following combination has correct options