You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   

 

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

 

Is there a second STS at which you would like to sequence PCR products obtained using BACs as templates? If so, which BACs would you test in this way, and what sequencing results might you predict for each of the BACs tested?

#Question id: 622

#SCPH05 I Biotechnology

An enzyme-catalyzed reaction was carried out with the substrate concentration initially a thousand times greater than the Km for that substrate.  After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12 micromol.  If, in a separate experiment, one-third as much enzyme and twice as much substrate had been combined, how long would it take for the same amount (12 micromol) of product to be formed?

#Question id: 18220

#SCPH01 Biochemistry

#Question id: 11275

#SCPH28 | Zoology

Consumption efficiency is the proportion of production at one trophic level that is eaten by, or ingested, by the next trophic level which of the following are correct statement ?

A.Consumption efficiencies higher in  forests than grassland

B-In aquatic ecosystems consuption efficiencies of hervivore are greater than terrestrial hervivore

C- Consumption efficiencies for carnivores are typically higher than those of herbivores

D-Primary production consumption is increased where it is high in nutrients such as nitrogen and phosphorus

#Question id: 694

#SCPH05 I Biotechnology

Thr and/or Leu residues tend to disrupt an alpha helix when they occur next to each other in a protein because:

#Question id: 4977

#SCPH28 | Zoology

There are at least a dozen known species in the extinct genus Lystrosaurus. If each species was suited to a quite different environment, then this relatively large number of species is likely due to