TLS Online TPP Program

#Question id: 16136


You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
Is there a second STS at which you would like to sequence PCR products obtained using BACs as templates? If so, which BACs would you test in this way, and what sequencing results might you predict for each of the BACs tested?

#SCPH06 I Botany
  1. STS1 is present more than once; BACs A, B,  would have the same sequence C, E, F would have the same sequence.
  2. STS5 is present more than once; BACs B, C, D, E, and F would have the same sequence.
  3. STS1 is present more than once; BACs A, B, C would have the same sequence E, F would have the same sequence.
  4. STS2 is present more than once; BACs B, C, D would have the different sequence.
More Questions
TLS Online TPP Program

#Question id: 9201

#SCPH28 | Zoology

Erwin and Scott used an insecticidal fog to knock down insects from the top section of a L. seemannii tree. The researchers identified over 900 species of beetles among the individuals that fell. Erwin also projected that this entire tree from top to bottom is host to about 600 arthropod species that are unique to this tree species and not found on any other tree species. There are approximately 50,000 species of tropical trees. Although it could not be entirely accurate, what would be the best way to estimate the total number of arthropod species?

TLS Online TPP Program

#Question id: 9202

#SCPH06 I Botany

Which of the following statements regarding extinction is accurate?

TLS Online TPP Program

#Question id: 9202

#SCPH28 | Zoology

Which of the following statements regarding extinction is accurate?

TLS Online TPP Program

#Question id: 9203

#SCPH06 I Botany

The human impact that scientists determined has caused the most extinctions on record is ________, and beyond further losses from this impact, the other impact with the largest potential to cause future extinctions is ________.

TLS Online TPP Program

#Question id: 9203

#SCPH28 | Zoology

The human impact that scientists determined has caused the most extinctions on record is ________, and beyond further losses from this impact, the other impact with the largest potential to cause future extinctions is ________.

TLS Online TPP Program

#Question id: 9204

#SCPH06 I Botany

Which of the following observations provides the best evidence of a biodiversity crisis?