Practice Questions

  1. Home
  2. Free Resources
  3. Practice Questions
  4. 46602
TLS Online TPP Program

#Question id: 16137

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
How would you use the sequence information presented in to design two new STSs (with new PCR primer pairs) to replace STS5? (Call the new ones STS51 and STS52. STS51 should be present (+) in BAC B, and STS52 should be present (+) in BAC F.)

#SCPH06 I Botany
  1. Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS52  not  STS51.
  2. Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify both simultaneously  STS51 and  STS52.
  3. Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS51  not  STS52.
  4. Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify either STS51 or STS52.
More Questions
TLS Online TPP Program

#Question id: 9210

#SCPH28 | Zoology

Overharvesting encourages extinction and is most likely to affect ________.
TLS Online TPP Program

#Question id: 621

#SCPH05 I Biotechnology

Michaelis and Menten assumed that the overall reaction for an enzyme-catalyzed reaction could be written as Using this reaction, the rate of breakdown of the enzyme-substrate complex can be described by the expression:
TLS Online TPP Program

#Question id: 9285

#SCPH06 I Botany

Non-O2-evolving (anoxygenic) organisms, contain only a single photosystem such as
TLS Online TPP Program

#Question id: 11654

#SCPH28 | Zoology

Succession start from the water body and continuos in community dyanamics, changes species composition as well as changes in structure also. Which one is the correct composition of hydrosere process?
TLS Online TPP Program

#Question id: 3647

#SCPH01 Biochemistry

In the cell cycle: there are four main phases, G1, S, G2 and M phase. S phase is the :