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TLS Online TPP Program

#Question id: 16138

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
Would you expect STS51 and STS52 to be present in BAC D? 

#SCPH06 I Botany
  1. Yes, STS51 and STS52 should both be present in BAC D
  2. No, STS51 and STS52 should not be present in BAC D, it will be present in BAC B only
  3. Yes, only  STS51 should be present in BAC D
  4. Yes STS51 and STS52 should both be present in BAC B, C, D, E and F
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TLS Online TPP Program

#Question id: 12909

#SCPH01 Biochemistry

According to the diagram, arrange the steps in correct order: 


1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.
TLS Online TPP Program

#Question id: 5219

#SCPH28 | Zoology

What is it that can be duplicated in a genome?
TLS Online TPP Program

#Question id: 10545

#SCPH05 I Biotechnology

Some given facts regarding effector molecules such as;

a) HC-toxin-induced plasma membrane hyperpolarization in guard cells causes massive K+ uptake and permanent stomatal opening

b) Xanthomonas, are proteins that target the plant cell nucleus and causes change in gene expression, called transcription activator-like (TAL) effectors bind to the host plant DNA and activate the expression of genes

c) Fusicoccin, a cyclic peptide, acts on the enzyme histone deacetylase in the nucleus, and may have a compromising effect on the expression of genes involved in defense

d) Gibberellins accelerate growth, resulting in bigger plants when compared with uninfested plants. The gibberellins produced by the fungi are identical to those produced endogenously by the plant

Which of the following combination is incorrect?
TLS Online TPP Program

#Question id: 13030

#SCPH06 I Botany

The mutation in Vir F will cause the interference in –
TLS Online TPP Program

#Question id: 3893

#SCPH01 Biochemistry

Protein structural motifs often have general functions in common.  Which one of the following motifs is known to be involved in protein dimer formation, but not in direct protein-DNA interactions?