During your study of the gene for an enzyme you have isolated an amber mutation in the middle of the gene which produces a truncated form of the enzyme. By placing a +1 frameshift mutation a short distance before the amber mutation and a –1 frameshift mutation a short distance after the amber mutation you create a triple mutant that restores expression of a functional, full-length enzyme. 

a) +1 frameshift puts the amber mutation out of frame so that it is no longer read as a stop.

b) -1 frameshift is inserted after the amber so that no longer read as a stop.

c)  -1 frameshift is inserted after the amber to restore the frame of the end of the protein.

d) +1 frameshift puts the amber mutation out of frame so that it restore the frame of the end of the protein.

Which of the following is the correct prediction about the frame shift mutation?

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