TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 1635
#I Life Science/ Life Sciences Group – I-V
What is immunological unresponsiveness to self-antigens called?
TLS Online TPP Program
#Question id: 15868
#SCPH05 I Biotechnology
If the culture is kept in stationary phase for a prolonged period, the cells may die. Why?
a) due to the time taken for doubling of cell number
b) duration of a cell generation is too large, depending mainly on the plant species.
c) stationary phase is forced on the culture by a depletion of the nutrients
d) due to an accumulation of cellular wastes
TLS Online TPP Program
#Question id: 2324
#SCPH06 I Botany
Bacterial DNA is located in:
TLS Online TPP Program
#Question id: 349
#SCPH05 I Biotechnology
Increased atmospheric CO2 concentrations might have what effect on seawater?
TLS Online TPP Program
#Question id: 13087
#SCPH06 I Botany
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?