In stimulated emission depletion (STED) microscopy the focused excitation laser point is surrounded by a 

TLS Online TPP Program

#Question id: 404

#SCPH01 Biochemistry

When a mixture of 3-phosphoglycerate and 2-phosphoglycerate is incubated at 25 °C with phosphoglycerate mutase until equilibrium is reached, the final mixture contains six times as much 2-phosphoglycerate as 3-phosphoglycerate.  Which one of the following statements is most nearly correct, when applied to the reaction as written?  (R = 8.315 J/mol·K; T = 298 K)  3-Phosphoglycerate -> 2-phosphoglycerate

TLS Online TPP Program

#Question id: 19353

#SCPH01 Biochemistry

Gene uidA encodes the enzyme P-glucourinidase which acts on the substrate 

TLS Online TPP Program

#Question id: 13059

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

TLS Online TPP Program

#Question id: 745

#SCPH01 Biochemistry

The structure of RNA differs from that of DNA, as RNA contains:

TLS Online TPP Program

#Question id: 5229

#SCPH28 | Zoology

Two eukaryotic proteins have one domain in common but are otherwise very different. Which of the following processes is most likely to have contributed to this phenomenon?