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TLS Online TPP Program
#Question id: 13093
#SCPH28 | Zoology
You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?
In this table, DV = digested vector. DYG = digested yeast genome.
TLS Online TPP Program
#Question id: 28000
#Research Methodology
Which of the following are the objectives of research?
a) Are not stated in first chapter of thesis
b) Answer to question through the application of scientific procedure
c) To find out the truth which is hidden
d) It is stated in first chapter of the thesis
TLS Online TPP Program
#Question id: 19123
#SCPH28 | Zoology
What is a Biosensor?
1. Device which employs a biological material to specifically interact with an analyte
2. Physical change measured and converted into an electrical signal
3. Device which separate analytes
Which of the following above statements are true for biosensor?
TLS Online TPP Program
#Question id: 10974
#I Life Science/ Life Sciences Group – I-V
A 63-year-old woman returned to work following a vacation in New Zealand. Several days after returning home, she awoke with swelling and pain in her right leg and her leg was blue. She went immediately to the emergency room where an examination showed an extensive deep vein thrombosis involving the femoral and iliac veins on the right side. Following resolution of the clot, this patient will require which treatment in the future?
TLS Online TPP Program
#Question id: 2958
#SCPH28 | Zoology
Which of the following structures is not a feature of the mitotic chromosome?