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TLS Online TPP Program
#Question id: 13060
#SCPH28 | Zoology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
In blue white screening insertional activation of Beta-D-galactosidase will give rise to
TLS Online TPP Program
#Question id: 4910
#SCPH28 | Zoology
For a trait known to be caused by a mitochondrial gene variant, there may be an occasional female that can transmit different phenotypes to her progeny on an apparently random basis. What situation might you suspect?
TLS Online TPP Program
#Question id: 3924
#SCPH28 | Zoology
Characteristics of ligands involved in transcription include:
TLS Online TPP Program
#Question id: 12933
#SCPH01 Biochemistry
Choose the correct order for the cloning process.
1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.
2. mRNA cannot be used directly in cloning.
3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.
4. The cDNA may be inserted into vectors and then cloned.
TLS Online TPP Program
#Question id: 11000
#I Life Science/ Life Sciences Group – I-V
What is her ventricular ejection fraction?