TLS Online TPP Program
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TLS Online TPP Program
#Question id: 13092
#SCPH01 Biochemistry
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?
TLS Online TPP Program
#Question id: 5567
#SCPH01 Biochemistry
The cortical reaction of sea urchin eggs functions directly in ________.
TLS Online TPP Program
#Question id: 10672
#SCPH28 | Zoology
Which of the following is incorrect match of protective strategy done by organism?
TLS Online TPP Program
#Question id: 444
#SCPH05 I Biotechnology
Which of the following is not a factor contributing to the large free energy of hydrolysis of ATP?
TLS Online TPP Program
#Question id: 18636
#SCPH05 I Biotechnology
Number of rooted trees in a phylogeny of three sequences is –