Approximately ________ base pairs are added per second during DNA in vivo replication in E.coli.

#Question id: 14162

#SCPH05 I Biotechnology

A continuous process is set up for treatment of wastewater. Each day, 10⁵ kg cellulose and 10³ kg bacteria enter in the feed stream, while 10⁴ kg cellulose and 1.5 x 10⁴ kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 7 x 10⁴ kg d^-1. The rate of bacterial growth is 2 x 10⁴ kg d^-1 ; the rate of cell death by lysis is 5 x 10² kg d^-1. Write balances for cellulose and bacteria in the system.

#Question id: 14163

#SCPH05 I Biotechnology

A stream of growth medium containing 60% glucose and 40% growth factor A flowing at 500 kg per hour was divided into two feed streams for different cell cultures. One fourth of the growth medium was sent to fermenter 1 and the rest was sent to fermenter 2. What is the flow rates of glucose and growth factor A in the feed streams for the two fermenters

#Question id: 14164

#SCPH05 I Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C_4.4H_7.30 _1.2N_0.86 . These bacteria are grown under aerobic conditions with hexadecane (C₁₆H₃₄) as substrate. The reaction equation describing growth is:

  Assuming 100% conversion, what is the yield of cells from hexadecane in g g^-l?   

#Question id: 14165

#SCPH05 I Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

Assuming 100% conversion, what is the yield of cells from oxygen in g g-l?

#Question id: 14166

#SCPH05 I Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium? 

#Question id: 14167

#SCPH05 I Biotechnology

Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest can be considered solids. Beet molasses containing 50% sucrose, 1% invert sugars, 18% water and the remainder solids, is mixed with corn-steep liquor in a mixing tank. Water is added to produce a diluted sugar mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor and 45 kg molasses are fed into the tank.

 How much water is required?