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#Question id: 13081
#SCPH05 I Biotechnology
Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.
TLS Online TPP Program
#Question id: 13081
#SCPH06 I Botany
Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.
TLS Online TPP Program
#Question id: 13081
#SCPH28 | Zoology
Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.
TLS Online TPP Program
#Question id: 13082
#SCPH01 Biochemistry
Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.
TLS Online TPP Program
#Question id: 13082
#SCPH05 I Biotechnology
Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.
TLS Online TPP Program
#Question id: 13082
#SCPH06 I Botany
Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.