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TLS Online TPP Program

#Question id: 361

Calculate the percentage of histidine that has its imidazole side chain protonated at pH 7.3. The pKa values for histidine are pK1 = 1.8, pK2 (imidazole) = 6.0, and pK3 = 9.2.

#SCPH28 | Zoology

  1. 90%

  2. 95.2%

  3. 89.4%

  4. 5%

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TLS Online TPP Program

#Question id: 10355

#SCPH06 I Botany

The conversion of ammonium to amino acids requires two enzymes such as GS and GOGAT,

a) High levels of light and carbohydrate—conditions that stimulate plastid GS and Fd-GOGAT

b) Conditions of ample energy inhibit GS and GOGAT

c) The stimulation of GS and GOGAT, they favor nitrogen assimilation into glutamine and glutamate, compounds that are rich in carbon and participate in the synthesis of new plant materials

d) In energy-limited conditions inhibit GS and GOGAT

Which of the following statements about GS and GOGAT is correct?
TLS Online TPP Program

#Question id: 2935

#SCPH28 | Zoology

Breakdown of the nuclear envelope during mitosis is accomplished by CDK-dependent phosphorylation of lamins:
TLS Online TPP Program

#Question id: 5751

#SCPH28 | Zoology

A biochemical mutant that must be supplied with a particular nutrient for growth would be described as a(n)
TLS Online TPP Program

#Question id: 13090

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 3’s plasmid is;
TLS Online TPP Program

#Question id: 1570

#SCPH06 I Botany

Which cells and which signaling molecules are responsible for initiating an inflammatory response?