Gene expression is often assayed by measuring the level of mRNA produced from a gene. What level of the control of gene expression can by analyzed by this type of assay?

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#Question id: 14136

#SCPH05 I Biotechnology

Which of the following statement is incorrect about global alignment? 

(A) Global alignment is based on the Needleman–Wunsch algorithm.

(B) In Global alignment algorithm, an optimal alignment is obtained over the entire lengths of the two sequences.

(C) It is only suitable for aligning two closely related sequences that are of the same length.

(D) GAP is a web-based pairwise global alignment program.

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#Question id: 7002

#SCPH28 | Zoology

The nematode Caenorhabditis elegans ________.

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#Question id: 346

#SCPH05 I Biotechnology

The partial negative charge in a molecule of water occurs because ________.

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#Question id: 2692

#SCPH05 I Biotechnology

 With respect to microsatellite DNA which of the following is correct?

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#Question id: 13057

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?